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apc anti mouse cd3 flow cytometry  (TargetMol)


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    TargetMol apc anti mouse cd3 flow cytometry
    Fig. 5. In sepsis, HMGB1 and TLR2 regulate the expression of PD-1 on T lymphocytes to mediate the occurrence of immune suppression. (A) Representative double- staining immunofluorescence of T Lymphocytes <t>(CD3)</t> with PD-1 in Sham, CLP, and CLP + GA mice (n = 3, scale = 50 μm). (B) Flow sorting of spleen T lymphocytes (n = 3). (C) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + GA groups (n = 3). (D) Representative double-staining immunofluorescence of T Lymphocytes <t>(CD3)</t> with PD-1 in Sham, CLP and CLP + C29 mice (n = 3, scale = 100 μm). (E) Flow sorting of spleen T lymphocytes (n = 3). (F) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + C29 groups (n = 4). Glycyrrhizic acid (GA) is an HMGB1 inhibitor; C29 is a TLR2 inhibitor. Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.
    Apc Anti Mouse Cd3 Flow Cytometry, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apc anti mouse cd3 flow cytometry/product/TargetMol
    Average 92 stars, based on 1 article reviews
    apc anti mouse cd3 flow cytometry - by Bioz Stars, 2026-05
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    1) Product Images from "HMGB1 promotes neutrophil PD-L1 expression through TLR2 and mediates T cell apoptosis leading to immunosuppression in sepsis."

    Article Title: HMGB1 promotes neutrophil PD-L1 expression through TLR2 and mediates T cell apoptosis leading to immunosuppression in sepsis.

    Journal: International immunopharmacology

    doi: 10.1016/j.intimp.2024.112130

    Fig. 5. In sepsis, HMGB1 and TLR2 regulate the expression of PD-1 on T lymphocytes to mediate the occurrence of immune suppression. (A) Representative double- staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP, and CLP + GA mice (n = 3, scale = 50 μm). (B) Flow sorting of spleen T lymphocytes (n = 3). (C) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + GA groups (n = 3). (D) Representative double-staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP and CLP + C29 mice (n = 3, scale = 100 μm). (E) Flow sorting of spleen T lymphocytes (n = 3). (F) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + C29 groups (n = 4). Glycyrrhizic acid (GA) is an HMGB1 inhibitor; C29 is a TLR2 inhibitor. Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.
    Figure Legend Snippet: Fig. 5. In sepsis, HMGB1 and TLR2 regulate the expression of PD-1 on T lymphocytes to mediate the occurrence of immune suppression. (A) Representative double- staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP, and CLP + GA mice (n = 3, scale = 50 μm). (B) Flow sorting of spleen T lymphocytes (n = 3). (C) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + GA groups (n = 3). (D) Representative double-staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP and CLP + C29 mice (n = 3, scale = 100 μm). (E) Flow sorting of spleen T lymphocytes (n = 3). (F) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + C29 groups (n = 4). Glycyrrhizic acid (GA) is an HMGB1 inhibitor; C29 is a TLR2 inhibitor. Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.

    Techniques Used: Expressing, Double Immunofluorescence Staining, Western Blot



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    Fig. 5. In sepsis, HMGB1 and TLR2 regulate the expression of PD-1 on T lymphocytes to mediate the occurrence of immune suppression. (A) Representative double- staining immunofluorescence of T Lymphocytes <t>(CD3)</t> with PD-1 in Sham, CLP, and CLP + GA mice (n = 3, scale = 50 μm). (B) Flow sorting of spleen T lymphocytes (n = 3). (C) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + GA groups (n = 3). (D) Representative double-staining immunofluorescence of T Lymphocytes <t>(CD3)</t> with PD-1 in Sham, CLP and CLP + C29 mice (n = 3, scale = 100 μm). (E) Flow sorting of spleen T lymphocytes (n = 3). (F) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + C29 groups (n = 4). Glycyrrhizic acid (GA) is an HMGB1 inhibitor; C29 is a TLR2 inhibitor. Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.
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    Image Search Results


    Fig. 5. In sepsis, HMGB1 and TLR2 regulate the expression of PD-1 on T lymphocytes to mediate the occurrence of immune suppression. (A) Representative double- staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP, and CLP + GA mice (n = 3, scale = 50 μm). (B) Flow sorting of spleen T lymphocytes (n = 3). (C) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + GA groups (n = 3). (D) Representative double-staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP and CLP + C29 mice (n = 3, scale = 100 μm). (E) Flow sorting of spleen T lymphocytes (n = 3). (F) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + C29 groups (n = 4). Glycyrrhizic acid (GA) is an HMGB1 inhibitor; C29 is a TLR2 inhibitor. Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.

    Journal: International immunopharmacology

    Article Title: HMGB1 promotes neutrophil PD-L1 expression through TLR2 and mediates T cell apoptosis leading to immunosuppression in sepsis.

    doi: 10.1016/j.intimp.2024.112130

    Figure Lengend Snippet: Fig. 5. In sepsis, HMGB1 and TLR2 regulate the expression of PD-1 on T lymphocytes to mediate the occurrence of immune suppression. (A) Representative double- staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP, and CLP + GA mice (n = 3, scale = 50 μm). (B) Flow sorting of spleen T lymphocytes (n = 3). (C) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + GA groups (n = 3). (D) Representative double-staining immunofluorescence of T Lymphocytes (CD3) with PD-1 in Sham, CLP and CLP + C29 mice (n = 3, scale = 100 μm). (E) Flow sorting of spleen T lymphocytes (n = 3). (F) Western blot compared the expression of PD-1 on T lymphocytes in sham, CLP and CLP + C29 groups (n = 4). Glycyrrhizic acid (GA) is an HMGB1 inhibitor; C29 is a TLR2 inhibitor. Data are expressed as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ns means no significance.

    Article Snippet: T lymphocytes sorted by APC anti-Mouse CD3 flow cytometry was lysed with RIPA lysis buffer (GBCBIO) containing 1 mM PMSF (Ubio), 1 × phosphatase inhibitor cocktail (GLPBIO), and 1 × EDTA-free protease inhibitor cocktail (TargetMol).

    Techniques: Expressing, Double Immunofluorescence Staining, Western Blot

    Journal: iScience

    Article Title: The immune checkpoint ICOSLG is a relapse-predicting biomarker and therapeutic target in infant t(4;11) acute lymphoblastic leukemia

    doi: 10.1016/j.isci.2022.104613

    Figure Lengend Snippet:

    Article Snippet: Mouse Anti-Human CD3, monoclonal antibody, APC-H7 conjugated (flow cytometry) , BD , BD Biosciences Cat# 560176, RRID: AB_1645475.

    Techniques: Recombinant, Flow Cytometry, Neutralization, Functional Assay, Western Blot, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software, Fluorescence, Marker